Hey, I’m Adrika, a fourth-year undergrad at IISER Kolkata. I am majoring in Biology and minoring in Physics. I like behavioral neurobiology, modelling different systems, and studying how behavior changes with context to learning and social interaction. Currently, I work with Drosophila melanogaster in Dr. Mohit Prasad’s Lab, and my project deals with post mating responses in mated females. I have also developed a simulation modelling border cell migration (I hope to write more about my projects some day), and am currently working on a theoretical ecology project as well.
I have also recently started dabbling in Philosphy as well, though I do not know enough to comment about it. I enjoy discussing philosophy with some friends I have come across.
In my free time, I like hiking, birdwatching, literature, and cooking. I enjoy writing about things I come across.
Thanks for dropping by!
Just a cheatsheet for protein expression and cloning:
Expression Vectors Feature | pET | pBAD | pQE | pGEX Promoter | T7 (requires T7 polymerase) | AraBAD | T5 | Tac Inducible Expression | IPTG | Arabinose | IPTG | IPTG Fusion Tags | His-tag (6x) | His-tag (6x) | His-tag (6x) | GST (Glutathione-S-transferase) Advantages | High-level expression | Tight control, low basal expression | High-level expression | Solubility and purification with GST tag Disadvantages | Inclusion bodies, requires special host | Less efficient than IPTG induction | Inclusion bodies | GST tag can affect protein function Best For | High-expression, large-scale protein production | Toxic proteins, low basal expression | High-yield protein production | Protein interaction studies, solubility issues
DNA Watson and Crick’s hypothesis had immediately suggested a possible self-replication method for DNA because of complementarity in base pairing.
At this junction, possible modes of DNA replication: Conservative / Semi-conservative / Dispersive
Meselson-Stahl Experiment proves replication is semi-conservative Bacteria grown in N(15) medium incorporate N14 in a light medium - CsCl density gradient centrifugation revealed.
Identification of Replication Fork John Cairns identified the replication fork by allowing replicating DNA in cells to incorporate tritiated thymidine. Each daughter molecule contains a radioactive strand and a cold strand.
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Regulation
Q. Why don't somatic cells have telomerases? They prevent the accummulation of harmful mutations in this way.