Border Cell Migration
What is migration? The conversion of stationary epithelial cells into migratory, invasive cells, which is important for normal embryonic development. How do cells achieve this? Cells that are part of a stable epithelium lose their cell-cell and cell-matrix junctions that hold them together and acquire a less rigidly polarised morphology and break away from their neighbours. Forward genetic analysis This involves uncovering the genetic basis behind a particular phenotype. Mutant lines are screened for specific defects in the process of interest. Molecular, cellular and chemical characterisation of the affected genes and proteins is then carried out. Reverse genetics: manipulating genes and studying their effect on phenotypes ...