Just a cheatsheet for protein expression and cloning:

Expression Vectors

Feature | pET | pBAD | pQE | pGEX Promoter | T7 (requires T7 polymerase) | AraBAD | T5 | Tac Inducible Expression | IPTG | Arabinose | IPTG | IPTG Fusion Tags | His-tag (6x) | His-tag (6x) | His-tag (6x) | GST (Glutathione-S-transferase) Advantages | High-level expression | Tight control, low basal expression | High-level expression | Solubility and purification with GST tag Disadvantages | Inclusion bodies, requires special host | Less efficient than IPTG induction | Inclusion bodies | GST tag can affect protein function Best For | High-expression, large-scale protein production | Toxic proteins, low basal expression | High-yield protein production | Protein interaction studies, solubility issues